Effect of Sample Preparation on Antibacterial Activity of Etlingera pavieana (Pierre ex Gagnep.) R.M.S. Extracts Against Foodborne Bacteriap
บทคัดย่อการประชุมทางวิชาการ ครั้งที่ 53 กลุ่มที่ 2 สาขาอุตสาหกรรมเกษตร
|c3-6 กุมภาพันธ์ 2558
The aim of this study was to determine the antibacterial activity of Etlingera pavieana extracts obtained by different sample preparation methods including parts of plant (leave, stem and rhizome), drying methods (oven and freeze drying) and various solvents (ethanol, acetone, dichloromethane, ethyl acetate, petroleum ether and hexane). Antibacterial activity was determined using the agar well diffusion method against foodborne microorganisms. The extractive yields of all extracts from oven drying were higher than freeze drying. The highest extractive yields were in ethyl acetate extract, followed by petroleum ether and hexane, respectively. All extracts showed antibacterial activity against all tested microorganisms. Gram-positive bacteria were more sensitive than Gram-negative bacteria. All extracts from oven drying showed higher antibacterial activity than freeze drying. Antibacterial activity of ethyl acetate extracted from leave was higher than stem and rhizome. Antibacterial activity of E. paviana in different solvent extracts showed different selectivity for each microorganism. However, petroleum ether extracts had the highest antibacterial activity against all tested bacteria except V. parahaemolyticus, with inhibition zone ranging from 15.5-42 mm. The preliminary studies on E. pavieana extracts showed their antimicrobial potential, which could be exploited further as future antibacterial agent for food preservation.
บทคัดย่อการประชุมทางวิชาการ ครั้งที่ 53 ของมหาวิทยาลัยเกษตรศาสตร์
Book of Abstracts Kasetsart Annual Conference