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Author Sirisansaneeyakul, Sarote
TitleEnzymatic production of fructo-oligosaccharides from sucrose/Sarote Sirisansaneeyakul ... [et al.]
Call# T030299

CALL # Volume Number Month Year Barcode Status

AbstractTo increase an efficiency of fructo-oligosaccharide production from sucrose, a mixed-enzyme system of B-fructofuranosidase (Aspergillus niger ATCC 20611)and glucose oxidase (Sigma Chemical Co., Ltd.)was carried out in a 2-1 stirred tank reactor with the following controlled conditions; temperature 40 degree C, pH 5.5, aeration rate 1 vvm and agitation rate 550 rpm, for 32 hours of incubation time. With an initial sucrose concentration of 400 g/l, the optimal condition was obtained by using B-fructofuranosidase and glucose oxidase 10 and 15 units per gram sucrose, respectively. As a result, the yields of kestose (Y GF2/S)and nystose (Y GF3/S)from sucrose and their volumetric productivities (Q GF2/S and Q GF3/S)were 0.44, 0.49 g/g, and 4.97, 5.44 g/l h, respectively.
Descriptvol.34 no.2 ; 262-269
Aspergillus niger