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Author Pongjanta, Jirapa
Alternative Author Kasetsart University
TitleEnzymes-resistant starch (RS III)from Pullulanase-debranched high amylose rice starch/Jirapa Pongjanta ... [et al.]
Alternative Titleสตาร์ชที่ทนต่อเอนไซม์ (RS III)จากการดัดแปรสตาร์ชข้าวแอมิโลสสูงด้วยเอนไซม์พูลูลาเนส
ImprintBangkok : Kasetsart University, 2008
Call# FOOD 6332

CALL # Volume Number Month Year Barcode Status

JournalThe Proceedings of 46th Kasetsart University Annual Conference; No.7, Subject: Agro-Industry, 29 January - 1 February 2008 / Organized by Kasetsart University ... [et al.]
AbstractIn this studied, a debranching enzyme (Pullulanase, EC. 232-983-9P, 8U/g starch at 55 degree C for 0 - 48-hr)was introduced to modify the amylopectin molecules of 15% (w/w)high amylose rice starches (32.10%, amylose content)which were partially gelatinized at 75 degree C for 30 min. The retrogradations of debranched starches with different degrees of hydrolysis (0.14-1.55%)were then induced at 4 degree C for 16 hrs. Afterwards, the freeze-thaw cycle (-10/30 degree C)was further applied to promote syneresis of retrograded starches. It was shown that pullulanase hydrolysis improved the degree of retrograded from 28.10 to 54.53%. The resistant starch content of pullulanase debranched starch increased from 4.80% to 12.33% by 0 to 48-hr, respectively. Results had showed that after debranching, starch molecule have rearranged and changed their crystal pattern from A to V-type revealed by X-ray diffraction analysis.
Descript677 p. ; 99-108
Subjectrice starch
Resistant starch
High amylose rice starch
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