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Author Hathaigan Kokkaew
Alternative Author Supawan Thawornchinsombu
Jae Won Park
Songklanakarin Journal of Science and Technology
TitleOptimal condition to remove mercury in yellowfin tuna protein isolates and ACE-inhibitory property of peptide prepared using commercial proteases
ImprintVol. 38 No. 4, July-August 2016
Call# FOOD 7777

CALL # Volume Number Month Year Barcode Status

AbstractResponse surface methodology (RSM) was performed to maximize the mercury (Hg) reduction from yellowf in tuna ( Thunnus albacare ) by products protein isolates (YBPI). The optimal condition of Hg reduction (89.3%) was 10.5 mM CaC l 2 and a Water:YB of 12.9:1, while other variables were fixed at 5 mM citric acid, 60 min incubation, p H 11 and 8,000 x g for 15 min of centrifugation. At these conditions, the significant protein recovery (80.1%) was obtained. H ydrolysates sequentially hydrolyzed with G6 followed by GN exhibited the highest angiotensin I-converting enzyme (ACE) inhibi tory activity than other enzyme preparations. Fractionated yellowfin tuna by products protein hydrolysate (YBPH) increa sed in ACE-inhibitory activity when peptide size decreased. In-vitro gastrointestinal (GI) digestion significantly increased bioactive property. ACE- inhibitory activity of YBPH with and without simulated GI digestion significantly increased after in cubating against ACE, demonstrating pro-drug type peptides.
DescriptPage 439-447
Subjectresponse surface methodology
commercial enzymes
pro-drug type peptides
gastrointestinal digestion