KOHA: OPAC Catalogue Search
Start OverMARC DisplayBrief DisplayReturn To BrowseExport Marked Records

Author Podchamarn Pimpakan
Alternative Author Wichien Yongmanitchai
Savitree Limtong
Kasetsart Journal Natural Science
TitleBioethanol Production from Sugar Cane Syrup by Thermo-tolerant Yeast, Kluyveromyces marxianus DMKU3-1042, using Fed-batch and Repeated-batch Fermentation in a Nonsterile System
ImprintVol. 46 No. 4, July-August 2012
Call# Food 7918

CALL # Volume Number Month Year Barcode Status

AbstractEthanol production from sugar cane syrup was carried out to compare the ef fi ciency of batch-, fed-batch and repeated-batch fermentation using Kluyveromyces marxianus DMKU3-1042. The sugar cane syrup medium contained 18% total sugar, 0.1% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 , and 0.1% MgSO 4 .7H 2 O, with the pH adjusted to 4.5. Experiments were carried out in a 2.5 L jar fermenter at a controlled temperature of 35 ? C and an agitation speed of 300 rpm without aeration. All experiments were carried out in a nonsterile system that resembled the process used in the bioethanol fermentation industry in Thailand. The ethanol concentration from batch fermentation was lowest among the three processes where 6.35% w/v was achieved in 72 hr resulting in an ethanol productivity of 0.88 g.L -1 . hr -1 and the ethanol yield corresponded to 82.73% of the theoretical yield. For fed-batch fermentation, the exponential feeding scheme barely improved the ethanol yields while sigmoidal feeding gave a considerably higher fi nal ethanol concentration reaching 7.42% w/v which was equivalent to ethanol productivity at 1.03 g.L -1 .hr -1 and 90.40% of theoretical yield. In the repeated-batch experiment, a rather high ethanol concentration of 7.91% w/v at 72 hr was obtained in the fi rst batch with ethanol productivity of 1.10 g.L -1 .hr -1 and 93.40% of the theoretical yield. However, the fi nal ethanol contents of the two successive batches were relatively low at 6.01% w/v and 6.66% w/v, respectively. The results from this research demonstrated that in Thailand, sugar cane syrup could be employed as an alternative renewable carbon source for ethanol production using sigmoidal fed-batch fermentation with an ef fi cient thermo- tolerant yeast strain ( Kluyveromyces marxianus DMKU3-1042).
DescriptPage 582-591
Subjectethanol production
sugar cane syrup
thermo-tolerant yeast
Kluyveromyces marxianus DMKU3-1042
fed-batch fermentation
repeated-batch fermentation