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Author Chutintorn Chauywong
Alternative Author Warapond Wanna
Amornrat Phongdara
Sasithorn Boonprakong
วารสารวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยสงขลานครินทร์
TitleStructural features and thermal stability of shrimp 14-3-3
Alternative TitleStructural features and thermal stability of shrimp 14-3-3
ImprintVol. 39 No. 4, July - August 2017
Call# FOOD 8337

CALL # Volume Number Month Year Barcode Status

AbstractIn our previous study, we reported that the nucleotide sequence of a 14-3-3? cDNA clone from Litopenaeus vannamei encodes two transcripts, designated as 14-3-3EL and 14-3-3ES. Based on the retained introns we observed in 14-3-3EL by alternative splicing, we aimed to investigate the genomic structure of this gene. The 14-3-3? gene, encoded by 2,311 bp of DNA sequence, is interrupted by three introns of 48, 1,124 and 365 bp in size. In addition, using the SWISS-MODEL, the predicted 3D model of 14-3-3EL has twelve ?-helices and two ?-sheets, whereas 14-3-3ES has only eleven helices in its tertiary structure. Additionally, we generated the 14-3-3EL and 14-3-3ES proteins and measured their thermal stabilities.The results demonstrated that the quantity of the 14-3-3EL protein decreased significantly at all temperatures tested. In contrast, the quantity of the 14-3-3ES protein remained unchanged, suggesting that 143-3-3EL is less stable than 14-3-3ES.
Descriptpage 531-537
Subjectalternative splicing
4-3-3 epsilon
beta-sheet
genomic structure
protein stability
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