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Author Kittithat Yongsirasawad
Alternative Author Patchanee Yasurin
KMUTNB: IJAST
TitleSimulated Gastrointestinal System Study of Centella asiatica Extract-loaded Gelatin Nanoparticles on Antioxidant and Antimicrobial Activities
Alternative TitleSimulated Gastrointestinal System Study of Centella asiatica Extract-loaded Gelatin Nanoparticles on Antioxidant and Antimicrobial Activities
ImprintVol. 10 No. 3, July - September 2017
Call# FOOD 8347

CALL # Volume Number Month Year Barcode Status

AbstractThe aim of this study is to improve antibacterial activity and antioxidant activity of Centella asiatica in simulated gastrointestinal system. Gelatin one-step and two-step desolvation nanoparticle methods were used to prepare C. asiatica-loaded gelatin nanoparticles (CGNP) on three different ratio of 95% ethanolic C. asiatica crude extracts:Gelatin (1:2,1:3,and 1:4). One-step CGNP (OSCGNP) was tested on antibacterial activity by using well agar diffusion method with different concentrations (100,200,and 300 /ml) against seven foodborne pathogens and antioxidant activity by using DPPH method. The inhibition zones of OSCGNP showed highly significant effective at concentration of 300 /ml in oesophagus-stomach section against E.coli ATCC25822 and B. subtilis respectively.In addition,S.aureus,S.enterica Enteritidis (human),and S.enterica 4,5,12:i:- (human) US clone were strongly inhibited by OSCGNP at concentration of 100/ml. The highest inhibition zone of OSCGNP was 1.00+-0.17 cm at PH 2.0 using gelatin one-step desolvation method. The highest antioxidant activity was 22.70+-4.69 ug GAE/ml per 10 mg of OSCGNP with ratio of 1:2 occurred in stomach at pH 2.0. Moreover,antioxidant activity of CGNPs (One-step and two-step gelatin nanoparticles) were dropped when they reached duodenum section.The results indicated that CGNPs gave lower antioxidant activity than crude extract.
DescriptPage 231-238
SubjectCentella asiatica
Simulated gastrointestinal system
Nanoparticle
Antibacterial activity
Antioxidant
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