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Author Peerada Prommeenate
Alternative Author Pavinee Kurdrit
Apiradee Hongsthong
Matura Sirijuntarut
Kasetsart Journal Natural Science
TitleExpression of Fatty Acid Desaturase Enzymes from Cyanobacterium Spirulina platensis in Yeast Saccharomyces cerevisiae
Alternative TitleExpression of Fatty Acid Desaturase Enzymes from Cyanobacterium Spirulina platensis in Yeast Saccharomyces cerevisiae
ImprintVol.41 No.1, January - March 2007
Call# FOOD 8369

CALL # Volume Number Month Year Barcode Status

AbstractSpirulina sp. is the cyanobacterium that has an ability to produce ?-linolenic acid or GLA at high level (1-1.5% of dry weight) which could be used as a new source of GLA production. The biosynthesis of GLA in Spirulina sp. involved with three enzymes, ?9, ?12 and ?6 desaturase which encoded by gene desC, desA and desD, respectively. The desaturase genes from Spirulina that involve in GLA biosynthesis from cyanobacteria were studied by gene cloning and transforming into yeast, Saccharomyces cerevisiae. Gas Chromatography has been used to follow the activity of desaturase enzymes in yeast cells. The results indicated that both ?12 and ?6 desaturase genes from Spirulina platenesis can functionally co-expressed in the heterologous yeast system. The final product, GLA has been detected under the condition described here.
DescriptPage 130-135
Subjectdesaturase
fatty acid desaturation
Spirulina platensis
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